Device

Part:BBa_K284022:Design

Designed by: Ane Fernanda Beraldi Zeidler, Luige A. Llerena Calderón and Marcos Henrique de Moraes   Group: iGEM09_UNICAMP-Brazil   (2009-10-14)

T4 endolysin under the control of T7 promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 322
    Illegal AgeI site found at 392
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For more accurate tests about the endolysin effectiveness, this part has to be under the control of an inducible promoter, like the T7 promoter.


Source

This device is a junction of previously designed parts: BBa_I746911 - Designed by Stefan Milde Group: iGEM07_Cambridge (2008-09-30) BBa_K112806 - Designed by Jin Huh Group: iGEM08_UC_Berkeley (2008-10-31) Bba_B0014 - Designed by Reshma Shetty Group: Registry (2003-07-16)

References

Callewaert, L.; Masschalck, B.; Deckers, D.; Nakimbugwe, D.; Atanassova, M.; Aertsen, A.; Michiels, C.W. 2005. Purification of Ivy, a lysozyme inhibitor from Escherichia coli, and characterization of its specificity for various lysozymes. Enzyme Microb. Technol. v. 37, 205-211.

Nakimbugwe, D.; Masschalck, B.; Deckers, D.; Callewaert, L.; Aertsen, A.; Michiels, C.W. 2006. Cell wall substrate specificity of six different lysozymes and lysozyme ihibitory activity of bacterial extracts. FEMS Microbiol. Lett. v. 259, 41-46.

Nakimbugwe, D.; Masschalck, B.; Atanassova, M.; Zewdie-Bosuener, A.; Michiels, C.W. 2006. Comparison of bactericidal activity of six lysozymes at atmospheric pressure and under high hydrostatic pressure. Int. J. Food Microbiol. v. 108, 355-363.